KMID : 1161519970010010093
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Animal Cells and Systems 1997 Volume.1 No. 1 p.93 ~ p.98
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Colocalization of ¥á-subunit of Gq protein with actin filaments in L8E63 cells
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Chae Sung-Suk
Park Dong-Eun
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Abstract
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The present study investigated the cellular localization of ¥á?subunit of Gq (G¥áq) protein in developing L8E63, rat skeletal muscle cell line. The colocalization of G¥áq with actin cytoskeleton was demonstrated by double?labeling experiments. In mononucleated myoblasts, the immune?fluorescence staining pattern of G¥áq was almost identical with that of F?actin visualized with rhodamine?conjugated phalloidin. However, this colocalization of G¥áq with cytoskeleton was not maintained in multinucleated myotubes. The staining pattern of G¥áq in myotubes did not match with any specific subcellular structure, but appeared as a uniformly distributed diffuse staining throughout the whole cell surface. Interestingly, change in the expression level of G¥áq was not detected during myoblast differentiation, suggesting that actin?associated G¥áq protein might dissociate from the cytoskeleton as cells differentiate. Immunocytochemical experiments using specific antibodies directed against several G proteins indicated that the subcellular localizations of G¥ái1, G¥ái2, G¥ái3, and G¥áo were different from those obtained with G¥áq.
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KEYWORD
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Gaq, F-actin, Colocalization, L8E63, Differentiation, Immunofluorescence
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